› 6jom

X-ray diffraction
2.45Å resolution

Crystal structure of lipoate protein ligase from Mycoplasma hyopneumoniae

Released:
DOI: 10.2210/pdb6jom/pdb
PDB entry 6jom coloured by chain, front view.
PDB entry 6jom coloured by chain, side view.
PDB entry 6jom coloured by chain, top view.
Source organism: Mycoplasma hyopneumoniae J
Primary publication:
Functional Identification and Structural Analysis of a New Lipoate Protein Ligase in Mycoplasma hyopneumoniae.
Zhu K, Chen H, Jin J, Wang N, Ma G, Huang J, Feng Y, Xin J, Zhang H, Liu H
Front Cell Infect Microbiol 10 156 (2020)
PMID: 32373550

Function and Biology Details

Reaction catalysed: 
(1a) ATP + (R)-lipoate = lipoyl-AMP + diphosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175435 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
lipoate--protein ligase Chains: A, B
Molecule details ›
Chains: A, B
Length: 351 amino acids
Theoretical weight: 40.84 KDa
Source organism: Mycoplasma hyopneumoniae J
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q4AA29 (Residues: 1-343; Coverage: 100%)
Gene names: MHJ_0302, lplA-1
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand LAQ 2 x LAQ
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U1, SSRF BEAMLINE BL19U1
Spacegroup: P41212
Unit cell:
a: 100.322Å b: 100.322Å c: 155.587Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.204 0.201 0.268
Expression system: Escherichia coli BL21(DE3)

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Citations

2 review citations

Infection strategies of mycoplasmas: Unraveling the panoply of virulence factors.
Yiwen et al. (2021)
1 more

2 mentions without citation

Functional Identification and Structural Analysis of a New Lipoate Protein Ligase in Mycoplasma hyopneumoniae.
Zhu et al. (2020)
1 more