› 6jpi

X-ray diffraction
3.14Å resolution

Crystal structure of PA4674 in complex with its operator DNA (28bp) from Pseudomonas aeruginosa

Released:
DOI: 10.2210/pdb6jpi/pdb
PDB entry 6jpi coloured by chain, front view.
PDB entry 6jpi coloured by chain, side view.
PDB entry 6jpi coloured by chain, top view.
Source organisms:
Entry authors: Liu Y, Gao Z, Zhang H, Dong Y

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-120091 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
HTH cro/C1-type domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 109 amino acids
Theoretical weight: 12.23 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: Q9HVC1 (Residues: 1-101; Coverage: 100%)
Gene name: PA4674
Sequence domains: Helix-turn-helix
DNA (28-MER) Chain: E
Molecule details ›
Chain: E
Length: 28 nucleotides
Theoretical weight: 8.52 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Not provided
DNA (28-MER) Chain: F
Molecule details ›
Chain: F
Length: 28 nucleotides
Theoretical weight: 8.68 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL18U1
Spacegroup: P212121
Unit cell:
a: 77.8Å b: 90.7Å c: 91.7Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.222 0.215 0.281
Expression systems:
  • Escherichia coli 'BL21-Gold(DE3)pLysS AG'
  • Not provided

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