› 6jqo

Electron Microscopy
3.1Å resolution

Structure of PaaZ, a bifunctional enzyme in complex with NADP+ and CCoA

Released:
DOI: 10.2210/pdb6jqo/pdb
PDB entry 6jqo coloured by chain, front view.
PDB entry 6jqo coloured by chain, side view.
PDB entry 6jqo coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Molecular basis for metabolite channeling in a ring opening enzyme of the phenylacetate degradation pathway.
Sathyanarayanan N, Cannone G, Gakhar L, Katagihallimath N, Sowdhamini R, Ramaswamy S, Vinothkumar KR
Nat Commun 10 4127 (2019)
PMID: 31511507
Related structures: EMD-9876

Function and Biology Details

Reactions catalysed: 
3-oxo-5,6-dehydrosuberyl-CoA semialdehyde + NADP(+) + H(2)O = 3-oxo-5,6-dehydrosuberyl-CoA + NADPH
2-oxepin-2(3H)-ylideneacetyl-CoA + H(2)O = 3-oxo-5,6-dehydrosuberyl-CoA semialdehyde
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160170 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bifunctional protein PaaZ Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 688 amino acids
Theoretical weight: 73.97 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P77455 (Residues: 2-681; Coverage: 100%)
Gene names: JW1382, b1387, maoC, paaZ, ydbN
Sequence domains:

Ligands and Environments


Cofactor: Ligand NAP 6 x NAP
1 bound ligand:
Ligand COO 6 x COO
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.1Å
Relevant EMDB volumes: EMD-9876
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Computational Methods for Single-Particle Electron Cryomicroscopy.
Singer et al. (2020)
2 more

3 mentions without citation

Bacterial Tropone Natural Products and Derivatives: Overview of their Biosynthesis, Bioactivities, Ecological Role and Biotechnological Potential.
Duan et al. (2020)
2 more