› 6jyw

X-ray diffraction
2.95Å resolution

Crystal structure of the transcription regulator CadR N81M mutant from P. putida in complex with Cadmium(II) and DNA

Released:
DOI: 10.2210/pdb6jyw/pdb
PDB entry 6jyw coloured by chain, front view.
PDB entry 6jyw coloured by chain, side view.
PDB entry 6jyw coloured by chain, top view.
Source organism: Pseudomonas putida
Entry authors: Liu XC, Chen H

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-120113 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
HTH merR-type domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 147 amino acids
Theoretical weight: 16.69 KDa
Source organism: Pseudomonas putida
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q93TP7 (Residues: 1-147; Coverage: 100%)
Gene names: BL240_26950, EFK07_13565, cadR
Sequence domains: MerR HTH family regulatory protein
DNA (5'-D(*AP*AP*CP*CP*CP*TP*AP*TP*AP*GP*TP*GP*GP*CP*TP*AP*CP*AP*GP*GP*GP*T)-3') Chain: C
Molecule details ›
Chain: C
Length: 22 nucleotides
Theoretical weight: 6.78 KDa
Source organism: Pseudomonas putida
Expression system: Not provided
DNA (5'-D(*TP*AP*CP*CP*CP*TP*GP*TP*AP*GP*CP*CP*AP*CP*TP*AP*TP*AP*GP*GP*GP*T)-3') Chain: D
Molecule details ›
Chain: D
Length: 22 nucleotides
Theoretical weight: 6.73 KDa
Source organism: Pseudomonas putida
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand CD 4 x CD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P63
Unit cell:
a: 126.435Å b: 126.435Å c: 93.783Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.205 0.202 0.253
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

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