› 6jz1

X-ray diffraction
1.73Å resolution

Apo structure of b-glucuronidase from Ruminococcus gnavus at 1.7 Angstrom resolution

Released:
DOI: 10.2210/pdb6jz1/pdb
Model geometry
Fit model/data
PDB entry 6jz1 coloured by chain, front view.
PDB entry 6jz1 coloured by chain, side view.
PDB entry 6jz1 coloured by chain, top view.
Source organism: [Ruminococcus] gnavus
Primary publication:
Substituent Position of Iminocyclitols Determines the Potency and Selectivity for Gut Microbial Xenobiotic-Reactivating Enzymes.
Dashnyam P, Lin HY, Chen CY, Gao S, Yeh LF, Hsieh WC, Tu Z, Lin CH
J Med Chem 63 4617-4627 (2020)
PMID: 32105467

Function and Biology Details

Reaction catalysed: 
A beta-D-glucuronoside + H(2)O = D-glucuronate + an alcohol
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-180486 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-glucuronidase Chains: A, B
Molecule details ›
Chains: A, B
Length: 603 amino acids
Theoretical weight: 70.04 KDa
Source organism: [Ruminococcus] gnavus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6W7J7 (Residues: 1-603; Coverage: 100%)
Gene name: uidA
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand MRD 3 x MRD
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: NSRRC BEAMLINE BL13C1
Spacegroup: C2
Unit cell:
a: 163.008Å b: 103.128Å c: 112.617Å
α: 90° β: 130.96° γ: 90°
R-values:
R R work R free
0.308 0.116 0.192
Expression system: Escherichia coli

Quick links

Citations

1 review citation

The Role of Gut Microbial β-Glucuronidase in Estrogen Reactivation and Breast Cancer.
Sui et al. (2021)
2 other articles

1 mention without citation

Ruminococcus gnavus: friend or foe for human health.
Crost et al. (2023)