› 6jzl

X-ray diffraction
2.32Å resolution

S-formylglutathione hydrolase homolog from a psychrophilic bacterium of Shewanella frigidimarina

Released:
DOI: 10.2210/pdb6jzl/pdb
PDB entry 6jzl coloured by chain, front view.
PDB entry 6jzl coloured by chain, side view.
PDB entry 6jzl coloured by chain, top view.
Source organism: Shewanella frigidimarina
Primary publication:
Structural and functional characterization of a novel cold-active S-formylglutathione hydrolase (SfSFGH) homolog from Shewanella frigidimarina, a psychrophilic bacterium.
Lee CW, Yoo W, Park SH, Le LTHL, Jeong CS, Ryu BH, Shin SC, Kim HW, Park H, Kim KK, Kim TD, Lee JH
Microb Cell Fact 18 140 (2019)
PMID: 31426813

Function and Biology Details

Reaction catalysed: 
S-formylglutathione + H(2)O = glutathione + formate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170582 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
S-formylglutathione hydrolase Chains: A, B
Molecule details ›
Chains: A, B
Length: 279 amino acids
Theoretical weight: 31.07 KDa
Source organism: Shewanella frigidimarina
Expression system: Escherichia coli
UniProt:
  • Canonical: Q07XK4 (Residues: 1-279; Coverage: 100%)
Gene name: Sfri_3426
Sequence domains: Putative esterase

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 5C (4A)
Spacegroup: P21
Unit cell:
a: 49.356Å b: 76.594Å c: 64.725Å
α: 90° β: 105.32° γ: 90°
R-values:
R R work R free
0.161 0.158 0.235
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Enzymes from Marine Polar Regions and Their Biotechnological Applications.
Bruno et al. (2019)
2 more

3 mentions without citation

Cold-Active Lipases and Esterases: A Review on Recombinant Overexpression and Other Essential Issues.
Matinja et al. (2022)
2 more