› 6k15

Electron Microscopy
3.4Å resolution

RSC substrate-recruitment module

Released:
DOI: 10.2210/pdb6k15/pdb
PDB entry 6k15 coloured by chain, front view.
PDB entry 6k15 coloured by chain, side view.
PDB entry 6k15 coloured by chain, top view.
Source organism: Saccharomyces cerevisiae S288C
Primary publication:
Structure of the RSC complex bound to the nucleosome.
Ye Y, Wu H, Chen K, Clapier CR, Verma N, Zhang W, Deng H, Cairns BR, Gao N, Chen Z
Science 366 838-843 (2019)
PMID: 31672915
Related structures: EMD-9905

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tridecamer (preferred)
PDBe Complex ID:
PDB-CPX-150527 (preferred)
Entry contents:
12 distinct polypeptide molecules
Macromolecules (12 distinct):
Chromatin structure-remodeling complex subunit RSC7 Chain: F
Molecule details ›
Chain: F
Length: 435 amino acids
Theoretical weight: 49.72 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P32832 (Residues: 1-435; Coverage: 100%)
Gene names: NPL6, RSC7, YM9582.16C, YMR091C
Sequence domains: Chromatin remodelling complex Rsc7/Swp82 subunit
Chromatin structure-remodeling complex protein RSC8 Chains: D, H
Molecule details ›
Chains: D, H
Length: 557 amino acids
Theoretical weight: 63.25 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P43609 (Residues: 1-557; Coverage: 100%)
Gene names: RSC8, SWH3, YFR037C
Sequence domains:
Chromatin structure-remodeling complex subunit RSC9 Chain: M
Molecule details ›
Chain: M
Length: 581 amino acids
Theoretical weight: 65.29 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: Q03124 (Residues: 1-581; Coverage: 100%)
Gene names: RSC9, YM4987.08, YML127W
Chromatin structure-remodeling complex protein RSC6 Chain: I
Molecule details ›
Chain: I
Length: 483 amino acids
Theoretical weight: 54.22 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P25632 (Residues: 1-483; Coverage: 100%)
Gene names: RSC6, YCR052W, YCR52W
Chromatin structure-remodeling complex subunit SFH1 Chain: G
Molecule details ›
Chain: G
Length: 426 amino acids
Theoretical weight: 48.83 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: Q06168 (Residues: 1-426; Coverage: 100%)
Gene names: L8543.4, SFH1, YLR321C
Sequence domains: SNF5 / SMARCB1 / INI1
Chromatin structure-remodeling complex protein RSC58 Chain: A
Molecule details ›
Chain: A
Length: 502 amino acids
Theoretical weight: 57.87 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: Q07979 (Residues: 1-502; Coverage: 100%)
Gene names: RSC58, YLR033W
Sequence domains: Bromodomain
Nuclear protein STH1/NPS1 Chain: J
Molecule details ›
Chain: J
Length: 1359 amino acids
Theoretical weight: 156.98 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P32597 (Residues: 1-1359; Coverage: 100%)
Gene names: NPS1, STH1, YIL126W
Sequence domains:
High temperature lethal protein 1 Chain: E
Molecule details ›
Chain: E
Length: 78 amino acids
Theoretical weight: 9.19 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: Q9URQ5 (Residues: 1-78; Coverage: 100%)
Gene names: DRT1, HTL1, YCR020W-B
Chromatin structure-remodeling complex protein RSC30 Chain: C
Molecule details ›
Chain: C
Length: 883 amino acids
Theoretical weight: 101.45 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P38781 (Residues: 1-883; Coverage: 100%)
Gene names: RSC30, YHR056C
Sequence domains: Fungal Zn(2)-Cys(6) binuclear cluster domain
Chromatin structure-remodeling complex protein RSC3 Chain: K
Molecule details ›
Chain: K
Length: 885 amino acids
Theoretical weight: 101.83 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: Q06639 (Residues: 1-885; Coverage: 100%)
Gene names: D9740.13, RSC3, YDR303C
Sequence domains: Fungal Zn(2)-Cys(6) binuclear cluster domain
Chromatin structure-remodeling complex subunit RSC4 Chain: X
Molecule details ›
Chain: X
Length: 625 amino acids
Theoretical weight: 72.37 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: Q02206 (Residues: 1-625; Coverage: 100%)
Gene names: RSC4, YK107, YKR008W
Sequence domains: Bromodomain
Chromatin structure-remodeling complex subunit RSC2 Chain: L
Molecule details ›
Chain: L
Length: 889 amino acids
Theoretical weight: 102.44 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: Q06488 (Residues: 1-889; Coverage: 100%)
Gene names: L9638.1, RSC2, YLR357W
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.4Å
Relevant EMDB volumes: EMD-9905

Quick links

Citations

13 review citations

Principles of nucleosome recognition by chromatin factors and enzymes.
McGinty et al. (2021)
12 more

26 mentions without citation

The paraflagellar rod of kinetoplastid parasites: from structure to components and function.
Portman et al. (2010)
25 more