› 6k1n

X-ray diffraction
2.26Å resolution

PLP-bound form of a putative cystathionine gamma-lyase

Released:
DOI: 10.2210/pdb6k1n/pdb
PDB entry 6k1n coloured by chain, front view.
PDB entry 6k1n coloured by chain, side view.
PDB entry 6k1n coloured by chain, top view.
Source organism: Stenotrophomonas maltophilia R551-3
Primary publication:
Structural characterization of cystathionine γ-lyase smCSE enables aqueous metal quantum dot biosynthesis.
Wang Y, Chen H, Huang Z, Yang M, Yu H, Peng M, Yang Z, Chen S
Int J Biol Macromol 174 42-51 (2021)
PMID: 33497694

Function and Biology Details

Reaction catalysed: 
(1a) L-cystathionine = L-cysteine + 2-aminobut-2-enoate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-110126 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cystathionine gamma-lyase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 392 amino acids
Theoretical weight: 41.77 KDa
Source organism: Stenotrophomonas maltophilia R551-3
Expression system: Escherichia coli
UniProt:
  • Canonical: B4SII9 (Residues: 1-390; Coverage: 100%)
Gene name: Smal_0489
Sequence domains: Cys/Met metabolism PLP-dependent enzyme

Ligands and Environments


Cofactor: Ligand PLP 4 x PLP
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U1
Spacegroup: P212121
Unit cell:
a: 90.025Å b: 141.735Å c: 157.833Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.19 0.188 0.242
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Cloneable inorganic nanoparticles.
Hendricks et al. (2023)
1 other article

1 mention without citation

Catalytic specificity of the Lactobacillus plantarum cystathionine γ-lyase presumed by the crystallographic analysis.
Matoba et al. (2020)