› 6k1t

X-ray diffraction
1.58Å resolution

The structure of Francisella virulence factor BioJ

Released:
DOI: 10.2210/pdb6k1t/pdb
PDB entry 6k1t coloured by chain, front view.
PDB entry 6k1t coloured by chain, side view.
PDB entry 6k1t coloured by chain, top view.
Source organism: Francisella philomiragia subsp. philomiragia ATCC 25015
Primary publication:
Molecular Basis of BioJ, a Unique Gatekeeper in Bacterial Biotin Synthesis.
Wei W, Guan H, Zhu T, Zhang S, Fan C, Ouyang S, Feng Y
iScience 19 796-808 (2019)
PMID: 31494495

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-111663 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alpha/beta hydrolase fold-3 domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 306 amino acids
Theoretical weight: 35.48 KDa
Source organism: Francisella philomiragia subsp. philomiragia ATCC 25015
Expression system: Escherichia coli
UniProt:
  • Canonical: C6YW90 (Residues: 1-306; Coverage: 100%)
Gene name: BZ13_192
Sequence domains: alpha/beta hydrolase fold

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P21
Unit cell:
a: 43.943Å b: 67.313Å c: 55.571Å
α: 90° β: 112.55° γ: 90°
R-values:
R R work R free
0.208 0.207 0.235
Expression system: Escherichia coli

Quick links

Citations

4 citation in other articles

Biochemical and structural characterization of the BioZ enzyme engaged in bacterial biotin synthesis pathway.
Zhang et al. (2021)
3 more

2 mentions without citation

Molecular Basis of BioJ, a Unique Gatekeeper in Bacterial Biotin Synthesis.
Wei et al. (2019)
1 more