6k2j

X-ray diffraction
2.4Å resolution

Crystal Structure of the DNA Complex of C. crescentus GapR

Released:
Primary publication:
GapR binds DNA through dynamic opening of its tetrameric interface.
Nucleic Acids Res 48 9372-9386 (2020)
PMID: 32756896

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-110723 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
UPF0335 protein CCNA_03428 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 97 amino acids
Theoretical weight: 11.2 KDa
Source organism: Caulobacter vibrioides NA1000
Expression system: Escherichia coli
UniProt:
  • Canonical: B8H4R9 (Residues: 1-89; Coverage: 100%)
Gene name: CCNA_03428
Sequence domains: GapR-like, DNA-binding domain
10A DNA_front Chain: E
Molecule details ›
Chain: E
Length: 16 nucleotides
Theoretical weight: 4.9 KDa
Source organism: Caulobacter vibrioides NA1000
Expression system: Not provided
10A DNA_reverse Chain: F
Molecule details ›
Chain: F
Length: 16 nucleotides
Theoretical weight: 4.89 KDa
Source organism: Caulobacter vibrioides NA1000
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U1
Spacegroup: P65
Unit cell:
a: 65.31Å b: 65.31Å c: 274.24Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.224 0.221 0.268
Expression systems:
  • Escherichia coli
  • Not provided