› 6k3c

X-ray diffraction
3.07Å resolution

Crystal structure of class I PHA synthase (PhaC) mutant from Chromobacterium sp. USM2 bound to Coenzyme A.

Released:
DOI: 10.2210/pdb6k3c/pdb
PDB entry 6k3c coloured by chain, front view.
PDB entry 6k3c coloured by chain, side view.
PDB entry 6k3c coloured by chain, top view.
Source organism: Chromobacterium sp. USM2
Primary publication:
Asymmetric Open-Closed Dimer Mechanism of Polyhydroxyalkanoate Synthase PhaC.
Chek MF, Kim SY, Mori T, Tan HT, Sudesh K, Hakoshima T
iScience 23 101084 (2020)
PMID: 32388399

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-122740 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Poly-beta-hydroxybutyrate polymerase N-terminal domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 395 amino acids
Theoretical weight: 43.83 KDa
Source organism: Chromobacterium sp. USM2
Expression system: Escherichia coli
UniProt:
  • Canonical: E1APK1 (Residues: 175-567; Coverage: 69%)
Gene name: phaC
Sequence domains: Poly-beta-hydroxybutyrate polymerase (PhaC) N-terminus

Ligands and Environments

1 bound ligand:
Ligand COA 1 x COA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: C2
Unit cell:
a: 169.802Å b: 61.852Å c: 82.566Å
α: 90° β: 106.73° γ: 90°
R-values:
R R work R free
0.226 0.224 0.263
Expression system: Escherichia coli

Quick links

Citations

2 review citations

A review on poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) [P(3HB-co-3HHx)] and genetic modifications that affect its production.
Tang et al. (2022)
1 more

1 mention without citation

Characterization and Homology Modeling of Catalytically Active Recombinant PhaCAp Protein from Arthrospira platensis.
Duangsri et al. (2023)