6k4p

X-ray diffraction
2.9Å resolution

Crystal structure of xCas9 in complex with sgRNA and DNA (TGG PAM)

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-120121 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
1 distinct RNA molecule
Macromolecules (4 distinct):
CRISPR-associated endonuclease Cas9/Csn1 Chain: B
Molecule details ›
Chain: B
Length: 1368 amino acids
Theoretical weight: 158.57 KDa
Source organism: Streptococcus pyogenes serotype M1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q99ZW2 (Residues: 1-1368; Coverage: 100%)
Gene names: SPy_1046, cas9, csn1
Sequence domains:
DNA (28-MER) Chain: C
Molecule details ›
Chain: C
Length: 28 nucleotides
Theoretical weight: 8.55 KDa
Source organism: Streptococcus pyogenes
Expression system: Not provided
non-targeted DNA Chain: D
Molecule details ›
Chain: D
Length: 12 nucleotides
Theoretical weight: 3.73 KDa
Source organism: Streptococcus pyogenes
Expression system: Not provided
sgRNA Chain: A
Molecule details ›
Chain: A
Length: 84 nucleotides
Theoretical weight: 27.09 KDa
Source organism: Streptococcus pyogenes
Expression system: Not provided

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: C2
Unit cell:
a: 177.785Å b: 69.667Å c: 189.016Å
α: 90° β: 109.52° γ: 90°
R-values:
R R work R free
0.206 0.203 0.269
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided