› 6k5m

X-ray diffraction
1.79Å resolution

The crystal structure of a serotonin N-acetyltransferase from Oryza Sativa (Rice)

Released:
DOI: 10.2210/pdb6k5m/pdb
PDB entry 6k5m coloured by chain, front view.
PDB entry 6k5m coloured by chain, side view.
PDB entry 6k5m coloured by chain, top view.
Source organism: Oryza sativa Japonica Group
Primary publication:
Structural and Molecular Dynamics Analysis of Plant Serotonin N-Acetyltransferase Reveal an Acid/Base-Assisted Catalysis in Melatonin Biosynthesis.
Liao L, Zhou Y, Xu Y, Zhang Y, Liu X, Liu B, Chen X, Guo Y, Zeng Z, Zhao Y
Angew Chem Int Ed Engl 60 12020-12026 (2021)
PMID: 33682300

Function and Biology Details

Reaction catalysed: 
Acetyl-CoA + a 2-arylethylamine = CoA + an N-acetyl-2-arylethylamine
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177346 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Serotonin N-acetyltransferase 1, chloroplastic Chain: A
Molecule details ›
Chain: A
Length: 171 amino acids
Theoretical weight: 19.51 KDa
Source organism: Oryza sativa Japonica Group
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5KQI6 (Residues: 92-254; Coverage: 64%)
Gene names: GNAT5, LOC_Os05g40260, NSI, OSJNBa0095J22.4, Os05g0481000, OsJ_018182, OsJ_18949, SNAT, SNAT1
Sequence domains: Acetyltransferase (GNAT) family

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 4 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL18U1
Spacegroup: P43212
Unit cell:
a: 66.893Å b: 66.893Å c: 82.692Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.226 0.225 0.234
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Melatonin-Mediated Abiotic Stress Tolerance in Plants.
Zeng et al. (2022)
2 more