› 6k7y

Electron Microscopy
3.6Å resolution

Intact human mitochondrial calcium uniporter complex with MICU1/MICU2 subunits

Released:
DOI: 10.2210/pdb6k7y/pdb
PDB entry 6k7y coloured by chain, front view.
PDB entry 6k7y coloured by chain, side view.
PDB entry 6k7y coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of intact human MCU supercomplex with the auxiliary MICU subunits.
Zhuo W, Zhou H, Guo R, Yi J, Zhang L, Yu L, Sui Y, Zeng W, Wang P, Yang M
Protein Cell 12 220-229 (2021)
PMID: 32862359
Related structures: EMD-9945

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero icosamer (preferred)
PDBe Complex ID:
PDB-CPX-184955 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Calcium uniporter protein, mitochondrial Chains: A, B, C, D, N, O, P, Q
Molecule details ›
Chains: A, B, C, D, N, O, P, Q
Length: 276 amino acids
Theoretical weight: 32.3 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q8NE86 (Residues: 73-348; Coverage: 79%)
Gene names: C10orf42, CCDC109A, MCU
Sequence domains: Mitochondrial calcium uniporter
Essential MCU regulator, mitochondrial Chains: E, F, G, H, R, S, T, U
Molecule details ›
Chains: E, F, G, H, R, S, T, U
Length: 54 amino acids
Theoretical weight: 5.99 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q9H4I9 (Residues: 48-101; Coverage: 51%)
Gene names: C22orf32, EMRE, SMDT1
Sequence domains: Putative mitochondrial precursor protein
Calcium uptake protein 1, mitochondrial Chains: I, V
Molecule details ›
Chains: I, V
Length: 476 amino acids
Theoretical weight: 54.43 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q9BPX6 (Residues: 1-476; Coverage: 100%)
Gene names: CALC, CBARA1, MICU1
Sequence domains: EF hand
Calcium uptake protein 2, mitochondrial Chains: J, W
Molecule details ›
Chains: J, W
Length: 336 amino acids
Theoretical weight: 39.85 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q8IYU8 (Residues: 83-418; Coverage: 77%)
Gene names: EFHA1, MICU2

Ligands and Environments

3 bound ligands:
Ligand CA 2 x CA
Ligand CDL 8 x CDL
Ligand PLX 16 x PLX
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.6Å
Relevant EMDB volumes: EMD-9945
Expression system: Homo sapiens

Quick links

Citations

9 review citations

Molecular nature and physiological role of the mitochondrial calcium uniporter channel.
Alevriadou et al. (2021)
8 more

3 mentions without citation

Structural characterization of the mitochondrial Ca2+ uniporter provides insights into Ca2+ uptake and regulation.
Wu et al. (2021)
2 more