› 6k9a

X-ray diffraction
2.3Å resolution

The complex of NrS-1 N terminal domain (1-305) with dGTP

Released:
DOI: 10.2210/pdb6k9a/pdb
PDB entry 6k9a coloured by chain, front view.
PDB entry 6k9a coloured by chain, side view.
PDB entry 6k9a coloured by chain, top view.
Source organism: Nitratiruptor phage NrS-1
Entry authors: Chen X, Gan J

Function and Biology Details

Reactions catalysed: 
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
ATP + H(2)O = ADP + phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-126037 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA Primase-polymerase Chain: A
Molecule details ›
Chain: A
Length: 306 amino acids
Theoretical weight: 34.86 KDa
Source organism: Nitratiruptor phage NrS-1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: M5AAG8 (Residues: 1-305; Coverage: 43%)
Gene name: 28

Ligands and Environments

2 bound ligands:
Ligand CA 2 x CA
Ligand DGT 1 x DGT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U1
Spacegroup: C2
Unit cell:
a: 119.114Å b: 58.007Å c: 47.135Å
α: 90° β: 92.96° γ: 90°
R-values:
R R work R free
0.211 0.209 0.248
Expression system: Escherichia coli BL21(DE3)

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