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› 6ke1

X-ray diffraction
3.39Å resolution

Crystal structure of TtCas1

Released:
DOI: 10.2210/pdb6ke1/pdb
Model geometry
Fit model/data
PDB entry 6ke1 coloured by chain, front view.
PDB entry 6ke1 coloured by chain, side view.
PDB entry 6ke1 coloured by chain, top view.
Source organism: Thermus thermophilus HB8
Primary publication:
Crystal structure of Cas1 in complex with branched DNA.
Yang J, Li J, Wang J, Sheng G, Wang M, Zhao H, Yang Y, Wang Y
Sci China Life Sci 63 516-528 (2020)
PMID: 31792780

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176177 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
CRISPR-associated endonuclease Cas1 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 325 amino acids
Theoretical weight: 35.77 KDa
Source organism: Thermus thermophilus HB8
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q53WG8 (Residues: 1-325; Coverage: 100%)
Gene names: TTHB193, cas1-2
Sequence domains: CRISPR associated protein Cas1

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SSRF BEAMLINE BL17U1
Spacegroup: P6422
Unit cell:
a: 150.432Å b: 150.432Å c: 219.43Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.221 0.221 0.229
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Structure-based insights into recognition and regulation of SAM-sensing riboswitches.
Zheng et al. (2023)
1 other article

3 mentions without citation

Current Efforts in the Development of Vaccines for the Prevention of Zika and Chikungunya Virus Infections.
Schrauf et al. (2020)
2 more