We've built a new version of this page with an interactive 3D viewer. Try it out in beta and help us improve:

› 6kew

X-ray diffraction
2.29Å resolution

Crystal structure of oxidized phosphoribulokinase from Arabidopsis thaliana

Released:
DOI: 10.2210/pdb6kew/pdb
Model geometry
Fit model/data
PDB entry 6kew coloured by chain, front view.
PDB entry 6kew coloured by chain, side view.
PDB entry 6kew coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
Photosynthetic Phosphoribulokinase Structures: Enzymatic Mechanisms and the Redox Regulation of the Calvin-Benson-Bassham Cycle.
Yu A, Xie Y, Pan X, Zhang H, Cao P, Su X, Chang W, Li M
Plant Cell 32 1556-1573 (2020)
PMID: 32102842

Function and Biology Details

Reaction catalysed: 
[D-ribulose 5-phosphate + ATP = D-ribulose 1,5-bisphosphate + ADP + H(+)., D-ribulose 5-phosphate + ATP = D-ribulose 1,5-bisphosphate + ADP + H(+).]
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150561 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphoribulokinase, chloroplastic Chains: A, B
Molecule details ›
Chains: A, B
Length: 359 amino acids
Theoretical weight: 40.5 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli
UniProt:
  • Canonical: P25697 (Residues: 47-395; Coverage: 88%)
Gene names: At1g32060, T12O21.4
Sequence domains: Phosphoribulokinase / Uridine kinase family

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: C2
Unit cell:
a: 80.784Å b: 50.315Å c: 194.783Å
α: 90° β: 91.81° γ: 90°
R-values:
R R work R free
0.241 0.241 0.259
Expression system: Escherichia coli

Quick links

Citations

9 review citations

Metabolons, enzyme-enzyme assemblies that mediate substrate channeling, and their roles in plant metabolism.
Zhang et al. (2021)
8 more