› 6kf1

X-ray diffraction
2Å resolution

Microbial Hormone-sensitive lipase- E53 mutant S162A

Released:
DOI: 10.2210/pdb6kf1/pdb
PDB entry 6kf1 coloured by chain, front view.
PDB entry 6kf1 coloured by chain, side view.
PDB entry 6kf1 coloured by chain, top view.
Source organism: Erythrobacter longus
Entry authors: Xiaochen Y, Zhengyang L, Xuewei X, Jixi L

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-100434 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Alpha/beta hydrolase fold-3 domain-containing protein Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 309 amino acids
Theoretical weight: 32.62 KDa
Source organism: Erythrobacter longus
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: A0A074MDU6 (Residues: 4-312; Coverage: 98%)
Gene name: EH31_02760
Sequence domains: alpha/beta hydrolase fold
Alpha/beta hydrolase fold-3 domain-containing protein Chain: D
Molecule details ›
Chain: D
Length: 310 amino acids
Theoretical weight: 32.74 KDa
Source organism: Erythrobacter longus
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: A0A074MDU6 (Residues: 3-312; Coverage: 99%)
Gene name: EH31_02760
Sequence domains: alpha/beta hydrolase fold

Ligands and Environments

6 bound ligands:
Ligand NPO 11 x NPO
Ligand 6NA 5 x 6NA
Ligand PGE 1 x PGE
Ligand EDO 1 x EDO
Ligand SO4 2 x SO4
Ligand MES 1 x MES
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL19U1
Unit cell:
a: 70.394Å b: 129.786Å c: 220.582Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.16 0.159 0.189
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

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