› 6kf5

X-ray diffraction
2.09Å resolution

Microbial Hormone-sensitive lipase E53 mutant I256L

Released:
DOI: 10.2210/pdb6kf5/pdb
PDB entry 6kf5 coloured by chain, front view.
PDB entry 6kf5 coloured by chain, side view.
PDB entry 6kf5 coloured by chain, top view.
Source organism: Erythrobacter longus
Entry authors: Yang X, Li ZY, Li J, Xu XW

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-100435 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alpha/beta hydrolase fold-3 domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 314 amino acids
Theoretical weight: 33.16 KDa
Source organism: Erythrobacter longus
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: A0A074MDU6 (Residues: 1-314; Coverage: 100%)
Gene name: EH31_02760
Sequence domains: alpha/beta hydrolase fold

Ligands and Environments

5 bound ligands:
Ligand D8F 4 x D8F
Ligand 6NA 1 x 6NA
Ligand EDO 3 x EDO
Ligand GOL 8 x GOL
Ligand 2FK 1 x 2FK
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL19U1
Unit cell:
a: 70.772Å b: 129.603Å c: 219.707Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.184 0.183 0.214
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

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