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› 6kgj

X-ray diffraction
1.8Å resolution

M1Q-hNTAQ1 C28S

Released:
DOI: 10.2210/pdb6kgj/pdb
Model geometry
Fit model/data
PDB entry 6kgj coloured by chain, front view.
PDB entry 6kgj coloured by chain, side view.
PDB entry 6kgj coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Use of the LC3B-fusion technique for biochemical and structural studies of proteins involved in the N-degron pathway.
Kim L, Kwon DH, Heo J, Park MR, Song HK
J Biol Chem 295 2590-2600 (2020)
PMID: 31919097

Function and Biology Details

Reaction catalysed: 
N-terminal L-glutaminyl-[protein] + H2O = N-terminal L-glutamyl-[protein]+ NH4(+).
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188529 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein N-terminal glutamine amidohydrolase Chains: A, B
Molecule details ›
Chains: A, B
Length: 202 amino acids
Theoretical weight: 23.21 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q96HA8 (Residues: 2-202; Coverage: 98%)
  • Best match: Q96HA8-2 (Residues: 1-142)
Gene names: C8orf32, NTAQ1, WDYHV1
Sequence domains: N-terminal glutamine amidase

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: PAL/PLS BEAMLINE 5C (4A)
Spacegroup: I222
Unit cell:
a: 95.638Å b: 105.779Å c: 140.169Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.212 0.211 0.223
Expression system: Escherichia coli

Quick links

Citations

2 review citations

How the ends signal the end: Regulation by E3 ubiquitin ligases recognizing protein termini.
Sherpa et al. (2022)
1 more

1 mention without citation

Use of the LC3B-fusion technique for biochemical and structural studies of proteins involved in the N-degron pathway.
Kim et al. (2020)