› 6ki0

X-ray diffraction
2Å resolution

Crystal Structure of Human ASC-CARD

Released:
DOI: 10.2210/pdb6ki0/pdb
PDB entry 6ki0 coloured by chain, front view.
PDB entry 6ki0 coloured by chain, side view.
PDB entry 6ki0 coloured by chain, top view.
Source organisms:
Primary publication:
Homotypic CARD-CARD interaction is critical for the activation of NLRP1 inflammasome.
Xu Z, Zhou Y, Liu M, Ma H, Sun L, Zahid A, Chen Y, Zhou R, Cao M, Wu D, Zhao W, Li B, Jin T
Cell Death Dis 12 57 (2021)
PMID: 33431827

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142603 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Apoptosis-associated speck-like protein containing a CARD; Maltose/maltodextrin-binding periplasmic protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 466 amino acids
Theoretical weight: 51.49 KDa
Source organisms: Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0AEX9 (Residues: 27-384; Coverage: 97%)
  • Canonical: Q9ULZ3 (Residues: 112-195; Coverage: 43%)
Gene names: ASC, CARD5, JW3994, PYCARD, TMS1, b4034, malE
Sequence domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC
Carbohydrate polymer
1 bound ligand:
Ligand SO4 3 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: C2
Unit cell:
a: 175.76Å b: 56.02Å c: 146.93Å
α: 90° β: 106.58° γ: 90°
R-values:
R R work R free
0.222 0.22 0.252
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 review citations

Molecular and structural aspects of gasdermin family pores and insights into gasdermin-elicited programmed cell death.
Zahid et al. (2021)
4 more

3 mentions without citation

Homotypic CARD-CARD interaction is critical for the activation of NLRP1 inflammasome.
Xu et al. (2021)
2 more