› 6ki6

X-ray diffraction
2.5Å resolution

Crystal structure of BCL11A in complex with gamma-globin -115 HPFH region

Released:
DOI: 10.2210/pdb6ki6/pdb
PDB entry 6ki6 coloured by chain, front view.
PDB entry 6ki6 coloured by chain, side view.
PDB entry 6ki6 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural insights into the recognition of γ-globin gene promoter by BCL11A.
Yang Y, Xu Z, He C, Zhang B, Shi Y, Li F
Cell Res 29 960-963 (2019)
PMID: 31467406

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-120152 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
B-cell lymphoma/leukemia 11A Chains: A, B
Molecule details ›
Chains: A, B
Length: 109 amino acids
Theoretical weight: 12.64 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9H165 (Residues: 731-835; Coverage: 13%)
Gene names: BCL11A, CTIP1, EVI9, KIAA1809, ZNF856
Sequence domains: Zinc finger, C2H2 type
DNA (5'-D(*AP*TP*AP*TP*TP*GP*GP*TP*CP*AP*AP*GP*G)-3') Chains: C, F
Molecule details ›
Chains: C, F
Length: 13 nucleotides
Theoretical weight: 4.03 KDa
Source organism: Homo sapiens
Expression system: Not provided
DNA (5'-D(*TP*CP*CP*TP*TP*GP*AP*CP*CP*AP*AP*TP*A)-3') Chains: D, E
Molecule details ›
Chains: D, E
Length: 13 nucleotides
Theoretical weight: 3.91 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand ZN 5 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL18U1
Spacegroup: P3221
Unit cell:
a: 59.555Å b: 59.555Å c: 213.367Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.209 0.207 0.251
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

11 citation in other articles

The HRI-regulated transcription factor ATF4 activates BCL11A transcription to silence fetal hemoglobin expression.
Huang et al. (2020)
10 more

4 mentions without citation

Structural insights into the recognition of γ-globin gene promoter by BCL11A.
Yang et al. (2019)
3 more