6kj6

Electron Microscopy
3.8Å resolution

cryo-EM structure of Escherichia coli Crl transcription activation complex

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero decamer (preferred)
PDBe Complex ID:
PDB-CPX-199493 (preferred)
Entry contents:
6 distinct polypeptide molecules
2 distinct DNA molecules
1 distinct RNA molecule
Macromolecules (9 distinct):
DNA-directed RNA polymerase subunit alpha Chains: A, B
Molecule details ›
Chains: A, B
Length: 329 amino acids
Theoretical weight: 36.56 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A7Z4 (Residues: 1-329; Coverage: 100%)
Gene names: JW3257, b3295, pez, phs, rpoA, sez
Sequence domains:
DNA-directed RNA polymerase subunit beta Chain: C
Molecule details ›
Chain: C
Length: 1342 amino acids
Theoretical weight: 150.82 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A8V2 (Residues: 1-1342; Coverage: 100%)
Gene names: JW3950, b3987, groN, nitB, rif, ron, rpoB, stl, stv, tabD
Sequence domains:
DNA-directed RNA polymerase subunit beta' Chain: D
Molecule details ›
Chain: D
Length: 1416 amino acids
Theoretical weight: 156.54 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A8T7 (Residues: 1-1407; Coverage: 100%)
Gene names: JW3951, b3988, rpoC, tabB
Sequence domains:
DNA-directed RNA polymerase subunit omega Chain: E
Molecule details ›
Chain: E
Length: 91 amino acids
Theoretical weight: 10.25 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A800 (Residues: 1-91; Coverage: 100%)
Gene names: JW3624, b3649, rpoZ
Sequence domains: RNA polymerase Rpb6
RNA polymerase sigma factor RpoS Chain: F
Molecule details ›
Chain: F
Length: 334 amino acids
Theoretical weight: 38.35 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P13445 (Residues: 1-330; Coverage: 100%)
Gene names: JW5437, appR, b2741, katF, nur, otsX, rpoS, sigS
Sequence domains:
Sigma factor-binding protein Crl Chain: J
Molecule details ›
Chain: J
Length: 137 amino acids
Theoretical weight: 16.01 KDa
Source organism: Escherichia coli ATCC 8739
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P24251 (Residues: 1-133; Coverage: 100%)
Gene names: JW0230, b0240, crl
Sequence domains: Sigma factor-binding transcriptional regulator Crl
DNA (51-MER) Chain: G
Molecule details ›
Chain: G
Length: 51 nucleotides
Theoretical weight: 15.8 KDa
DNA (51-MER) Chain: H
Molecule details ›
Chain: H
Length: 51 nucleotides
Theoretical weight: 15.8 KDa
RNA (5'-R(*CP*UP*CP*GP*A)-3') Chain: I
Molecule details ›
Chain: I
Length: 5 nucleotides
Theoretical weight: 1.55 KDa

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.8Å
Relevant EMDB volumes: EMD-0700
Expression system: Escherichia coli BL21(DE3)