› 6kkk

X-ray diffraction
3.5Å resolution

Crystal structure of Drug:Proton Antiporter-1 (DHA1) Family SotB, in the inward open conformation (H115A mutant)

Released:
DOI: 10.2210/pdb6kkk/pdb
PDB entry 6kkk coloured by chain, front view.
PDB entry 6kkk coloured by chain, side view.
PDB entry 6kkk coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Visualizing the nonlinear changes of a drug-proton antiporter from inward-open to occluded state.
Xiao Q, Sun B, Zhou Y, Wang C, Guo L, He J, Deng D
Biochem Biophys Res Commun 534 272-278 (2021)
PMID: 33280821

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151910 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sugar efflux transporter Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 423 amino acids
Theoretical weight: 45.42 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P31122 (Residues: 1-396; Coverage: 100%)
Gene names: JW1521, b1528, sotB, ydeA

Ligands and Environments

1 bound ligand:
Ligand BNG 3 x BNG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: C2
Unit cell:
a: 261.618Å b: 61.822Å c: 122.463Å
α: 90° β: 93.42° γ: 90°
R-values:
R R work R free
0.234 0.231 0.267
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

The Major Facilitator Superfamily and Antimicrobial Resistance Efflux Pumps of the ESKAPEE Pathogen Staphylococcus aureus.
Stephen et al. (2023)
1 more

1 mention without citation

Structural Insights into Transporter-Mediated Drug Resistance in Infectious Diseases.
Kim et al. (2021)