› 6klr

X-ray diffraction
2.21Å resolution

Crystal structure of human WIPI3 in complex with the WIR-peptide from ATG2A

Released:
DOI: 10.2210/pdb6klr/pdb
PDB entry 6klr coloured by chain, front view.
PDB entry 6klr coloured by chain, side view.
PDB entry 6klr coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Multi-site-mediated entwining of the linear WIR-motif around WIPI β-propellers for autophagy.
Ren J, Liang R, Wang W, Zhang D, Yu L, Feng W
Nat Commun 11 2702 (2020)
PMID: 32483132

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-174035 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Autophagy-related protein 2 homolog A; WD repeat domain phosphoinositide-interacting protein 3 Chains: A, B
Molecule details ›
Chains: A, B
Length: 350 amino acids
Theoretical weight: 38.46 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q2TAZ0 (Residues: 1374-1404; Coverage: 2%)
  • Canonical: Q5MNZ6 (Residues: 8-344; Coverage: 91%)
Gene names: ATG2A, KIAA0404, WDR45B, WDR45L, WIPI3
Sequence domains: PROPPIN

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: P21212
Unit cell:
a: 75.124Å b: 121.063Å c: 37.768Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.215 0.213 0.255
Expression system: Escherichia coli

Quick links

Citations

7 review citations

Autophagy in health and disease: From molecular mechanisms to therapeutic target.
Lu et al. (2022)
6 more

2 mentions without citation

A possible role for VPS13-family proteins in bulk lipid transfer, membrane expansion and organelle biogenesis.
Melia et al. (2022)
1 more