› 6klw

Electron Microscopy
2.9Å resolution

Complex structure of Iota toxin enzymatic component (Ia) and binding component (Ib) pore with long stem

Released:
DOI: 10.2210/pdb6klw/pdb
PDB entry 6klw coloured by chain, front view.
PDB entry 6klw coloured by chain, side view.
PDB entry 6klw coloured by chain, top view.
Source organism: Clostridium perfringens
Primary publication:
Cryo-EM structures reveal translocational unfolding in the clostridial binary iota toxin complex.
Yamada T, Yoshida T, Kawamoto A, Mitsuoka K, Iwasaki K, Tsuge H
Nat Struct Mol Biol 27 288-296 (2020)
PMID: 32123390
Related structures: EMD-0720

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175105 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
PA14 domain-containing protein Chains: A, B, C, D, E, F, G
Molecule details ›
Chains: A, B, C, D, E, F, G
Length: 666 amino acids
Theoretical weight: 74.38 KDa
Source organism: Clostridium perfringens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q46221 (Residues: 210-875; Coverage: 79%)
Sequence domains:
ADP ribosyltransferase domain-containing protein Chain: H
Molecule details ›
Chain: H
Length: 417 amino acids
Theoretical weight: 48.06 KDa
Source organism: Clostridium perfringens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q46220 (Residues: 42-454; Coverage: 91%)
Sequence domains: ADP-ribosyltransferase exoenzyme

Ligands and Environments

1 bound ligand:
Ligand CA 14 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.9Å
Relevant EMDB volumes: EMD-0720
Expression systems:
  • Escherichia coli
  • Escherichia coli BL21(DE3)

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Citations

5 review citations

The Buzz about ADP-Ribosylation Toxins from Paenibacillus larvae, the Causative Agent of American Foulbrood in Honey Bees.
Ebeling et al. (2021)
4 more