Function and Biology Details
Reaction catalysed:
Strict requirement for an Asp residue at position P1 and has a preferred cleavage sequence of Tyr-Val-Ala-Asp-|-.
Biochemical function:
Biological process:
Cellular component:
- not assigned
Structure analysis Details
Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151485 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Caspase-1 subunit p20
Molecule details ›
Chains: A, C
Length: 167 amino acids
Theoretical weight: 18.78 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
Sequence domains: Caspase domain
Length: 167 amino acids
Theoretical weight: 18.78 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical:
P29466 (Residues: 131-297; Coverage: 41%)
Sequence domains: Caspase domain
Caspase-1 subunit p10
Molecule details ›
Chains: B, D
Length: 88 amino acids
Theoretical weight: 10.26 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
Sequence domains: Caspase domain
Length: 88 amino acids
Theoretical weight: 10.26 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical: P29466 (Residues: 317-404; Coverage: 22%)
Sequence domains: Caspase domain
Gasdermin-D, C-terminal
Molecule details ›
Chains: E, F
Length: 198 amino acids
Theoretical weight: 21.25 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
Sequence domains: Gasdermin PUB domain
Length: 198 amino acids
Theoretical weight: 21.25 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical: P57764 (Residues: 283-480; Coverage: 41%)
Sequence domains: Gasdermin PUB domain
Ligands and Environments
No bound ligands
No modified residues
Experiments and Validation Details
X-ray source:
SSRF BEAMLINE BL17U
Spacegroup:
C2
Expression system: Escherichia coli BL21(DE3)
