› 6kn8

Electron Microscopy
4.8Å resolution

Structure of human cardiac thin filament in the calcium bound state

Released:
DOI: 10.2210/pdb6kn8/pdb
PDB entry 6kn8 coloured by chain, front view.
PDB entry 6kn8 coloured by chain, side view.
PDB entry 6kn8 coloured by chain, top view.
Source organisms:
Primary publication:
Cardiac muscle thin filament structures reveal calcium regulatory mechanism.
Yamada Y, Namba K, Fujii T
Nat Commun 11 153 (2020)
PMID: 31919429
Related structures: EMD-0729

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 29-mer (preferred)
PDBe Complex ID:
PDB-CPX-140677 (preferred)
Entry contents:
6 distinct polypeptide molecules
Macromolecules (6 distinct):
Actin, alpha skeletal muscle Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O
Length: 375 amino acids
Theoretical weight: 41.86 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: P68135 (Residues: 3-377; Coverage: 100%)
Gene names: ACTA, ACTA1
Sequence domains: Actin
Tropomyosin alpha-1 chain Chains: P, Q, W, X
Molecule details ›
Chains: P, Q, W, X
Length: 274 amino acids
Theoretical weight: 31.56 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P09493 (Residues: 11-284; Coverage: 97%)
Gene names: C15orf13, TMSA, TPM1
Sequence domains: Tropomyosin
Tropomyosin alpha-1 chain Chains: R, S, Y, Z
Molecule details ›
Chains: R, S, Y, Z
Length: 31 amino acids
Theoretical weight: 3.53 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P09493 (Residues: 1-29; Coverage: 10%)
Gene names: C15orf13, TMSA, TPM1
Sequence domains: Tropomyosin
Troponin T, cardiac muscle Chains: T, a
Molecule details ›
Chains: T, a
Length: 174 amino acids
Theoretical weight: 21.45 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P45379 (Residues: 109-282; Coverage: 58%)
Gene name: TNNT2
Sequence domains: Troponin
Troponin I, cardiac muscle Chains: U, b
Molecule details ›
Chains: U, b
Length: 126 amino acids
Theoretical weight: 14.43 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P19429 (Residues: 41-166; Coverage: 60%)
Gene names: TNNC1, TNNI3
Sequence domains: Troponin
Troponin C, slow skeletal and cardiac muscles Chains: V, c
Molecule details ›
Chains: V, c
Length: 160 amino acids
Theoretical weight: 18.29 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P63316 (Residues: 2-161; Coverage: 99%)
Gene names: TNNC, TNNC1
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand ADP 15 x ADP
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.8Å
Relevant EMDB volumes: EMD-0729
Expression system: Escherichia coli

Quick links

Citations

17 review citations

VEGF-A in Cardiomyocytes and Heart Diseases.
Braile et al. (2020)
16 more

29 mentions without citation

Troponin Revealed: Uncovering the Structure of the Thin Filament On-Off Switch in Striated Muscle.
Tobacman LS. (2021)
28 more