› 6kqk

Electron Microscopy
2.17Å resolution

323 K cryoEM structure of Sso-KARI in complex with Mg2+, NADH and CPD

Released:
DOI: 10.2210/pdb6kqk/pdb
PDB entry 6kqk coloured by chain, front view.
PDB entry 6kqk coloured by chain, side view.
PDB entry 6kqk coloured by chain, top view.
Source organism: Saccharolobus solfataricus
Primary publication:
Temperature-Resolved Cryo-EM Uncovers Structural Bases of Temperature-Dependent Enzyme Functions.
Chen CY, Chang YC, Lin BL, Huang CH, Tsai MD
J Am Chem Soc 141 19983-19987 (2019)
PMID: 31829582
Related structures: EMD-0753

Function and Biology Details

Reaction catalysed: 
(2R)-2,3-dihydroxy-3-methylbutanoate + NADP(+) = (2S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dodecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189048 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putative ketol-acid reductoisomerase 2 Chains: A, B, C, D, E, F, G, H, I, J, K, L
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L
Length: 333 amino acids
Theoretical weight: 37.23 KDa
Source organism: Saccharolobus solfataricus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q97YJ9 (Residues: 1-333; Coverage: 100%)
Gene names: SSO1322, ilvC-2, ilvC2
Sequence domains:

Ligands and Environments


Cofactor: Ligand NAI 12 x NAI
2 bound ligands:
Ligand MG 24 x MG
Ligand 9TY 12 x 9TY
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.17Å
Relevant EMDB volumes: EMD-0753
Expression system: Escherichia coli

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Citations

2 review citations

Advances in methods for atomic resolution macromolecular structure determination.
Thompson et al. (2020)
1 more