6ksl

X-ray diffraction
2.59Å resolution

Staphylococcus aureus lipase - S116A inactive mutant

Released:

Function and Biology Details

Reaction catalysed:
Triacylglycerol + H(2)O = diacylglycerol + a carboxylate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-102715 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
triacylglycerol lipase Chains: A, B
Molecule details ›
Chains: A, B
Length: 408 amino acids
Theoretical weight: 45.78 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A0U1MWF9 (Residues: 295-690; Coverage: 57%)
Gene names: BN1321_80040, lip

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44XU
Spacegroup: P6122
Unit cell:
a: 164.209Å b: 164.209Å c: 233.053Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.25 0.249 0.268
Expression system: Escherichia coli