› 6ksm

X-ray diffraction
2.23Å resolution

Staphylococcus aureus lipase -Orlistat complex

Released:
DOI: 10.2210/pdb6ksm/pdb
PDB entry 6ksm coloured by chain, front view.
PDB entry 6ksm coloured by chain, side view.
PDB entry 6ksm coloured by chain, top view.
Source organism: Staphylococcus aureus
Primary publication:
Crystal structure of pathogenic Staphylococcus aureus lipase complex with the anti-obesity drug orlistat.
Kitadokoro K, Tanaka M, Hikima T, Okuno Y, Yamamoto M, Kamitani S
Sci Rep 10 5469 (2020)
PMID: 32214208

Function and Biology Details

Reaction catalysed: 
Triacylglycerol + H(2)O = diacylglycerol + a carboxylate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-102715 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
triacylglycerol lipase Chains: A, B
Molecule details ›
Chains: A, B
Length: 408 amino acids
Theoretical weight: 45.8 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A0U1MWF9 (Residues: 295-690; Coverage: 57%)
Gene names: BN1321_80040, lip

Ligands and Environments

6 bound ligands:
Ligand ZN 2 x ZN
Ligand CA 2 x CA
Ligand DH9 2 x DH9
Ligand DAO 4 x DAO
Ligand BUA 2 x BUA
Ligand STE 1 x STE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44XU
Spacegroup: P4122
Unit cell:
a: 132.51Å b: 132.51Å c: 248.243Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.228 0.226 0.259
Expression system: Escherichia coli

Quick links

Citations

4 citation in other articles

Heat-Killed Enterococcus faecalis Prevents Adipogenesis and High Fat Diet-Induced Obesity by Inhibition of Lipid Accumulation through Inhibiting C/EBP-α and PPAR-γ in the Insulin Signaling Pathway.
Lee et al. (2022)
3 more

4 mentions without citation

New Aspects on Listeria monocytogenes ST5-ECVI Predominance in a Heavily Contaminated Cheese Processing Environment.
Muhterem-Uyar et al. (2018)
3 more