› 6ktq

X-ray diffraction
1.98Å resolution

Crystal structure of catalytic domain of homocitrate synthase from Sulfolobus acidocaldarius (SaHCS(dRAM)) in complex with alpha-ketoglutarate/Zn2+/CoA

Released:
DOI: 10.2210/pdb6ktq/pdb
PDB entry 6ktq coloured by chain, front view.
PDB entry 6ktq coloured by chain, side view.
PDB entry 6ktq coloured by chain, top view.
Source organism: Sulfolobus acidocaldarius DSM 639
Primary publication:
Involvement of subdomain II in the recognition of acetyl-CoA revealed by the crystal structure of homocitrate synthase from Sulfolobus acidocaldarius.
Suzuki T, Tomita T, Hirayama K, Suzuki M, Kuzuyama T, Nishiyama M
FEBS J 288 1975-1988 (2021)
PMID: 32897601

Function and Biology Details

Reaction catalysed: 
Acetyl-CoA + H(2)O + 2-oxoglutarate = (R)-2-hydroxybutane-1,2,4-tricarboxylate + CoA
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175556 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Homocitrate synthase Chains: A, B
Molecule details ›
Chains: A, B
Length: 412 amino acids
Theoretical weight: 45.71 KDa
Source organism: Sulfolobus acidocaldarius DSM 639
Expression system: Escherichia coli
UniProt:
  • Canonical: Q4J989 (Residues: 1-382; Coverage: 82%)
Gene name: Saci_1304
Sequence domains: HMGL-like

Ligands and Environments


Cofactor: Ligand COA 2 x COA
4 bound ligands:
Ligand ZN 2 x ZN
Ligand AKG 2 x AKG
Ligand SO4 8 x SO4
Ligand 1PE 1 x 1PE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-5A
Spacegroup: P3221
Unit cell:
a: 114.693Å b: 114.693Å c: 125.713Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.176 0.174 0.216
Expression system: Escherichia coli

Quick links

Citations

1 citations in other articles

Conformational interdomain flexibility in a bacterial α-isopropylmalate synthase is necessary for leucine biosynthesis.
Bai et al. (2023)

2 mentions without citation

Insight into de-regulation of amino acid feedback inhibition: a focus on structure analysis method.
Naz et al. (2023)
1 more