› 6kww

X-ray diffraction
3Å resolution

HslU from Staphylococcus aureus

Released:
DOI: 10.2210/pdb6kww/pdb
PDB entry 6kww coloured by chain, front view.
PDB entry 6kww coloured by chain, side view.
PDB entry 6kww coloured by chain, top view.
Source organism: Staphylococcus aureus subsp. aureus Mu50
Primary publication:
Cleavage-Dependent Activation of ATP-Dependent Protease HslUV from Staphylococcus aureus.
Jeong S, Ahn J, Kwon AR, Ha NC
Mol Cells 43 694-704 (2020)
PMID: 32694241

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159121 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ATP-dependent protease ATPase subunit HslU Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P, Q, R, S, T, U, V, W, X
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P, Q, R, S, T, U, V, W, X
Length: 481 amino acids
Theoretical weight: 54.03 KDa
Source organism: Staphylococcus aureus subsp. aureus Mu50
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P63796 (Residues: 1-467; Coverage: 100%)
Gene names: SAV1254, clpY, hslU
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 5C (4A)
Spacegroup: P21
Unit cell:
a: 146.618Å b: 189.602Å c: 215.813Å
α: 90° β: 92.617° γ: 90°
R-values:
R R work R free
0.235 0.235 0.279
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 citation in other articles

Armeniaspirol analogues with more potent Gram-positive antibiotic activity show enhanced inhibition of the ATP-dependent proteases ClpXP and ClpYQ.
Darnowski et al. (2022)
1 more

1 mention without citation

Cleavage-Dependent Activation of ATP-Dependent Protease HslUV from Staphylococcus aureus.
Jeong et al. (2020)