› 6kxr

X-ray diffraction
2.45Å resolution

Crystal structure of wild type Alp1U from the biosynthesis of kinamycins

Released:
DOI: 10.2210/pdb6kxr/pdb
PDB entry 6kxr coloured by chain, front view.
PDB entry 6kxr coloured by chain, side view.
PDB entry 6kxr coloured by chain, top view.
Source organism: Streptomyces ambofaciens ATCC 23877
Primary publication:
Mutation of an atypical oxirane oxyanion hole improves regioselectivity of the α/β-fold epoxide hydrolase Alp1U.
Zhang L, De BC, Zhang W, Mándi A, Fang Z, Yang C, Zhu Y, Kurtán T, Zhang C
J Biol Chem 295 16987-16997 (2020)
PMID: 33004437

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-102316 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
AB hydrolase-1 domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 339 amino acids
Theoretical weight: 37.05 KDa
Source organism: Streptomyces ambofaciens ATCC 23877
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A0K2AJY3 (Residues: 8-326; Coverage: 100%)
Gene names: SAM23877_0167, SAM23877_7506
Sequence domains: alpha/beta hydrolase fold

Ligands and Environments

1 bound ligand:
Ligand MLT 1 x MLT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U1
Spacegroup: P212121
Unit cell:
a: 97.09Å b: 101.58Å c: 117.42Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.172 0.169 0.234
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 citations in other articles

Flavin-enabled reductive and oxidative epoxide ring opening reactions.
De et al. (2022)

1 mention without citation

Mutation of an atypical oxirane oxyanion hole improves regioselectivity of the α/β-fold epoxide hydrolase Alp1U.
Zhang et al. (2020)