› 6l00

X-ray diffraction
1.75Å resolution

Crystal structure of the cell-wall binding domain (CBD) of endolysin LysIME-EF1

Released:
DOI: 10.2210/pdb6l00/pdb
PDB entry 6l00 coloured by chain, front view.
PDB entry 6l00 coloured by chain, side view.
PDB entry 6l00 coloured by chain, top view.
Source organism: Enterococcus phage IMEEF1
Primary publication:
Structural and functional insights into a novel two-component endolysin encoded by a single gene in Enterococcus faecalis phage.
Zhou B, Zhen X, Zhou H, Zhao F, Fan C, Perčulija V, Tong Y, Mi Z, Ouyang S
PLoS Pathog 16 e1008394 (2020)
PMID: 32176738

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-197439 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Peptidase C51 domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 70 amino acids
Theoretical weight: 8.17 KDa
Source organism: Enterococcus phage IMEEF1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: S5MRN1 (Residues: 168-237; Coverage: 30%)

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U1
Spacegroup: I422
Unit cell:
a: 59.88Å b: 59.88Å c: 87.01Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.225 0.223 0.244
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Phage-Encoded Endolysins.
Abdelrahman et al. (2021)
3 more

2 mentions without citation

Biflavonoid as potential 3-chymotrypsin-like protease (3CLpro) inhibitor of SARS-Coronavirus.
Hartini et al. (2021)
1 more