› 6l3a

X-ray diffraction
3Å resolution

Cytochrome P450 107G1 (RapN) with everolimus

Released:
DOI: 10.2210/pdb6l3a/pdb
Model geometry
Fit model/data
PDB entry 6l3a coloured by chain, front view.
PDB entry 6l3a coloured by chain, side view.
PDB entry 6l3a coloured by chain, top view.
Source organism: Streptomyces rapamycinicus NRRL 5491
Primary publication:
Structural insights into CYP107G1 from rapamycin-producing Streptomyces rapamycinicus.
Kim V, Lim YR, Lee I, Lee JH, Han S, Pham TV, Kim H, Lee R, Kang LW, Kim D
Arch Biochem Biophys 692 108544 (2020)
PMID: 32822639

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-100779 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cytochrome P450 Chains: A, B
Molecule details ›
Chains: A, B
Length: 404 amino acids
Theoretical weight: 45.13 KDa
Source organism: Streptomyces rapamycinicus NRRL 5491
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A0A0NSZ7 (Residues: 1-404; Coverage: 100%)
Gene name: D3C57_102975
Sequence domains: Cytochrome P450

Ligands and Environments

2 bound ligands:
Ligand HEM 2 x HEM
Ligand E53 1 x E53
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: PAL/PLS BEAMLINE 5C (4A)
Spacegroup: P21
Unit cell:
a: 63.028Å b: 126.142Å c: 70.099Å
α: 90° β: 116.46° γ: 90°
R-values:
R R work R free
0.246 0.241 0.336
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 citations in other articles

Pseudomonas aeruginosa cytochrome P450 CYP168A1 is a fatty acid hydroxylase that metabolizes arachidonic acid to the vasodilator 19-HETE.
Tooker et al. (2022)

1 mention without citation

Structure-Function Analysis of the Biotechnologically Important Cytochrome P450 107 (CYP107) Enzyme Family.
Padayachee et al. (2023)