› 6l80

X-ray diffraction
2Å resolution

Crystal structure of pombe Mod21 N-terminus and Mozart1

Released:
DOI: 10.2210/pdb6l80/pdb
PDB entry 6l80 coloured by chain, front view.
PDB entry 6l80 coloured by chain, side view.
PDB entry 6l80 coloured by chain, top view.
Source organism: Schizosaccharomyces pombe 972h-
Primary publication:
Promiscuous Binding of Microprotein Mozart1 to γ-Tubulin Complex Mediates Specific Subcellular Targeting to Control Microtubule Array Formation.
Huang TL, Wang HJ, Chang YC, Wang SW, Hsia KC
Cell Rep 31 107836 (2020)
PMID: 32610137

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-143226 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Gamma-tubulin complex subunit mod21 Chains: A, C
Molecule details ›
Chains: A, C
Length: 114 amino acids
Theoretical weight: 12.94 KDa
Source organism: Schizosaccharomyces pombe 972h-
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9UT52 (Residues: 1-109; Coverage: 16%)
Gene names: SPAC806.08c, mod21
Sequence domains: Gamma-Tubulin ring complex non-core subunit mod21, N-terminal
Mitotic-spindle organizing protein 1 Chains: B, D
Molecule details ›
Chains: B, D
Length: 100 amino acids
Theoretical weight: 11.31 KDa
Source organism: Schizosaccharomyces pombe 972h-
Expression system: Escherichia coli
UniProt:
  • Canonical: P0CF96 (Residues: 1-64; Coverage: 100%)
Gene names: SPAC9G1.15c, mzt1, tam4
Sequence domains: Mitotic-spindle organizing gamma-tubulin ring associated

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL13B1
Spacegroup: P43212
Unit cell:
a: 83.513Å b: 83.513Å c: 122.792Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.202 0.2 0.235
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Molecular insight into how γ-TuRC makes microtubules.
Thawani et al. (2021)
1 more