› 6l8i

X-ray diffraction
1.7Å resolution

Crystal structure of CYP97A3 mutant S290D/W300L/S304V

Released:
DOI: 10.2210/pdb6l8i/pdb
PDB entry 6l8i coloured by chain, front view.
PDB entry 6l8i coloured by chain, side view.
PDB entry 6l8i coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
Structural basis for plant lutein biosynthesis from α-carotene.
Niu G, Guo Q, Wang J, Zhao S, He Y, Liu L
Proc Natl Acad Sci U S A 117 14150-14157 (2020)
PMID: 32513704

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188145 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein LUTEIN DEFICIENT 5, chloroplastic Chain: A
Molecule details ›
Chain: A
Length: 519 amino acids
Theoretical weight: 58.43 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli
UniProt:
  • Canonical: Q93VK5 (Residues: 78-595; Coverage: 87%)
Gene names: At1g31800, CYP97A3, F5M6.19, LUT5
Sequence domains: Cytochrome P450

Ligands and Environments

1 bound ligand:
Ligand HEM 1 x HEM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: P212121
Unit cell:
a: 58.069Å b: 81.52Å c: 110.196Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.177 0.176 0.197
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Metabolic Engineering of Model Microorganisms for the Production of Xanthophyll.
Wang et al. (2023)
8 other articles

1 mention without citation

Structural basis for plant lutein biosynthesis from α-carotene.
Niu et al. (2020)