› 6lb7

X-ray diffraction
2.1Å resolution

Crystal structure of the Ca2+-free and Ca2+-bound MICU1-MICU2 complex

Released:
DOI: 10.2210/pdb6lb7/pdb
PDB entry 6lb7 coloured by chain, front view.
PDB entry 6lb7 coloured by chain, side view.
PDB entry 6lb7 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The structure of the MICU1-MICU2 complex unveils the regulation of the mitochondrial calcium uniporter.
Wu W, Shen Q, Zhang R, Qiu Z, Wang Y, Zheng J, Jia Z
EMBO J 39 e104285 (2020)
PMID: 32790952

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-184952 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Calcium uptake protein 1, mitochondrial Chains: A, C
Molecule details ›
Chains: A, C
Length: 377 amino acids
Theoretical weight: 43.08 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9BPX6 (Residues: 97-444; Coverage: 73%)
Gene names: CALC, CBARA1, MICU1
Sequence domains: EF hand
Calcium uptake protein 2, mitochondrial Chains: B, D
Molecule details ›
Chains: B, D
Length: 330 amino acids
Theoretical weight: 38.72 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8IYU8 (Residues: 84-406; Coverage: 74%)
Gene names: EFHA1, MICU2

Ligands and Environments

1 bound ligand:
Ligand CA 2 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: P212121
Unit cell:
a: 85.481Å b: 107.721Å c: 170.381Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.195 0.194 0.227
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

8 review citations

Mitochondrial calcium exchange in physiology and disease.
Garbincius et al. (2022)
7 more

1 mention without citation

The structure of the MICU1-MICU2 complex unveils the regulation of the mitochondrial calcium uniporter.
Wu et al. (2020)