› 6lbf

X-ray diffraction
3.25Å resolution

Crystal structure of FEM1B

Released:
DOI: 10.2210/pdb6lbf/pdb
PDB entry 6lbf coloured by chain, front view.
PDB entry 6lbf coloured by chain, side view.
PDB entry 6lbf coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Molecular basis for arginine C-terminal degron recognition by Cul2FEM1 E3 ligase.
Chen X, Liao S, Makaros Y, Guo Q, Zhu Z, Krizelman R, Dahan K, Tu X, Yao X, Koren I, Xu C
Nat Chem Biol 17 254-262 (2021)
PMID: 33398168

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193838 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein fem-1 homolog B Chains: A, B
Molecule details ›
Chains: A, B
Length: 356 amino acids
Theoretical weight: 39.46 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9UK73 (Residues: 1-356; Coverage: 57%)
Gene names: F1AA, FEM1B, KIAA0396
Sequence domains: Ankyrin repeats (3 copies)

Ligands and Environments

1 bound ligand:
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U1
Spacegroup: P4212
Unit cell:
a: 127.81Å b: 127.81Å c: 143.034Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.222 0.218 0.252
Expression system: Escherichia coli

Quick links

Citations

2 review citations

How the ends signal the end: Regulation by E3 ubiquitin ligases recognizing protein termini.
Sherpa et al. (2022)
1 more

1 mention without citation

Structural basis and regulation of the reductive stress response.
Manford et al. (2021)