› 6lc1

X-ray diffraction
3.12Å resolution

Structural basis of NR4A1 bound to the human pituitary proopiomelanocortin gene promoter

Released:
DOI: 10.2210/pdb6lc1/pdb
PDB entry 6lc1 coloured by chain, front view.
PDB entry 6lc1 coloured by chain, side view.
PDB entry 6lc1 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis of NR4A1 bound to the human pituitary proopiomelanocortin gene promoter.
Jiang L, Wei H, Yan N, Dai S, Li J, Qu L, Chen X, Guo M, Chen Z, Chen Y
Biochem Biophys Res Commun 523 1-5 (2020)
PMID: 31822342

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-120212 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Nuclear receptor subfamily 4immunitygroup A member 1 Chains: A, D, G, J
Molecule details ›
Chains: A, D, G, J
Length: 87 amino acids
Theoretical weight: 9.9 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P22736 (Residues: 265-351; Coverage: 15%)
Gene names: GFRP1, HMR, NAK1, NR4A1
Sequence domains: Zinc finger, C4 type (two domains)
DNA Chains: F, I
Molecule details ›
Chains: F, I
Length: 26 nucleotides
Theoretical weight: 7.97 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
DNA (25-MER) Chains: C, L
Molecule details ›
Chains: C, L
Length: 26 nucleotides
Theoretical weight: 8 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli

Ligands and Environments

1 bound ligand:
Ligand ZN 8 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U1
Spacegroup: P1
Unit cell:
a: 36.822Å b: 63.21Å c: 91.762Å
α: 109.98° β: 90.21° γ: 89.54°
R-values:
R R work R free
0.212 0.209 0.268
Expression system: Escherichia coli

Quick links

Citations

1 review citation

The Paradoxical Roles of Orphan Nuclear Receptor 4A (NR4A) in Cancer.
Safe et al. (2021)
3 other articles