› 6lfp

X-ray diffraction
3.31Å resolution

Cry3Aa protein for enzyme entrapment

Released:
DOI: 10.2210/pdb6lfp/pdb
PDB entry 6lfp coloured by chain, front view.
PDB entry 6lfp coloured by chain, side view.
PDB entry 6lfp coloured by chain, top view.
Source organism: Bacillus thuringiensis
Primary publication:
In Vivo Enzyme Entrapment in a Protein Crystal.
Heater BS, Yang Z, Lee MM, Chan MK
J Am Chem Soc 142 9879-9883 (2020)
PMID: 32407637

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141166 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pesticidal crystal protein Cry3Aa Chain: A
Molecule details ›
Chain: A
Length: 644 amino acids
Theoretical weight: 73.18 KDa
Source organism: Bacillus thuringiensis
Expression system: Bacillus thuringiensis
UniProt:
  • Canonical: P0A381 (Residues: 1-644; Coverage: 100%)
Gene names: bt13, cry3A, cry3Aa, cryC, cryIIIA(a), cryIIIa
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE TPS 05A
Spacegroup: C2221
Unit cell:
a: 116.35Å b: 132.61Å c: 102.89Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.159 0.157 0.209
Expression system: Bacillus thuringiensis

Quick links

Citations

2 review citations

Can (We Make) Bacillus thuringiensis Crystallize More Than Its Toxins?
Tetreau et al. (2021)
1 more

1 mention without citation

Treatment of ARS deficiencies with specific amino acids.
Kok et al. (2021)