› 6lig

X-ray diffraction
2.62Å resolution

Crystal structure of human Glutamate oxaloacetate transaminase 1 (GOT1) in complex with AH

Released:
DOI: 10.2210/pdb6lig/pdb
PDB entry 6lig coloured by chain, front view.
PDB entry 6lig coloured by chain, side view.
PDB entry 6lig coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Yan S, Sun WG, Zhang YH

Function and Biology Details

Reactions catalysed: 
L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate
L-cysteine + 2-oxoglutarate = 2-oxo-3-sulfanylpropanoate + L-glutamate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147889 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aspartate aminotransferase, cytoplasmic Chains: A, B
Molecule details ›
Chains: A, B
Length: 411 amino acids
Theoretical weight: 46.1 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P17174 (Residues: 3-413; Coverage: 100%)
Gene name: GOT1
Sequence domains: Aminotransferase class I and II

Ligands and Environments


Cofactor: Ligand PLP 1 x PLP
1 bound ligand:
Ligand EE6 1 x EE6
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: P21
Unit cell:
a: 64.87Å b: 90.42Å c: 74.05Å
α: 90° β: 91.87° γ: 90°
R-values:
R R work R free
0.217 0.213 0.266
Expression system: Escherichia coli

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