› 6lj3

X-ray diffraction
2Å resolution

full length ASFV dUTPase in complex with alpha,beta-iminodUTP and magnesium ion

Released:
DOI: 10.2210/pdb6lj3/pdb
Model geometry
Fit model/data
PDB entry 6lj3 coloured by chain, front view.
PDB entry 6lj3 coloured by chain, side view.
PDB entry 6lj3 coloured by chain, top view.
Source organism: African swine fever virus
Primary publication:
Structural comparisons of host and African swine fever virus dUTPases reveal new clues for inhibitor development.
Liang R, Wang G, Zhang D, Ye G, Li M, Shi Y, Shi J, Chen H, Peng G
OpenAccess logo J Biol Chem 296 100015
PMID: 33139328

Function and Biology Details

Reaction catalysed: 
dUTP + H2O = dUMP + diphosphate + H(+).
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-179185 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Deoxyuridine 5'-triphosphate nucleotidohydrolase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 165 amino acids
Theoretical weight: 18.29 KDa
Source organism: African swine fever virus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q65199 (Residues: 1-165; Coverage: 100%)
Gene names: Ba71V-131, E165R
Sequence domains: dUTPase

Ligands and Environments

2 bound ligands:
Ligand DUP 3 x DUP
Ligand MG 3 x MG
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: P212121
Unit cell:
a: 68.04Å b: 68.427Å c: 117.969Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.174 0.171 0.235
Expression system: Escherichia coli BL21(DE3)

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Citations

1 review citation

Viral dUTPases: Modulators of Innate Immunity.
Ariza et al. (2022)
3 other articles

3 mentions without citation

Structures and Functional Diversities of ASFV Proteins.
Wang et al. (2021)
2 more