› 6llz

X-ray diffraction
2.01Å resolution

Crystal Structure of Fagopyrum esculentum M UGT708C1 complexed with UDP-glucose

Released:
DOI: 10.2210/pdb6llz/pdb
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Source organism: Fagopyrum esculentum
Primary publication:
Crystal Structures of the C-Glycosyltransferase UGT708C1 from Buckwheat Provide Insights into the Mechanism of C-Glycosylation.
Liu M, Wang D, Li Y, Li X, Zong G, Fei S, Yang X, Lin J, Wang X, Shen Y
Plant Cell 32 2917-2931 (2020)
PMID: 32699169

Function and Biology Details

Reaction catalysed: 
UDP-alpha-D-glucose + a 2'-hydroxy-beta-oxodihydrochalcone = UDP + a 3'-(beta-D-glucopyranosyl)-2'-hydroxy-beta-oxodihydrochalcone
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-100803 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
UDP-glycosyltransferase 708C1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 457 amino acids
Theoretical weight: 50.43 KDa
Source organism: Fagopyrum esculentum
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A0A1HA03 (Residues: 1-457; Coverage: 100%)
Gene names: CGTa, UGT708C1
Sequence domains: UDP-glucoronosyl and UDP-glucosyl transferase

Ligands and Environments

1 bound ligand:
Ligand UPG 2 x UPG
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: P21212
Unit cell:
a: 98.914Å b: 143.757Å c: 69.758Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.17 0.168 0.202
Expression system: Escherichia coli

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Citations

4 citation in other articles

Glycosyltransferases: Mining, engineering and applications in biosynthesis of glycosylated plant natural products.
He et al. (2022)
3 more

1 mention without citation

Structure-function and engineering of plant UDP-glycosyltransferase.
Wang et al. (2023)

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