› 6lmj

X-ray diffraction
2.8Å resolution

ASFV pA104R in complex with double-strand DNA

Released:
DOI: 10.2210/pdb6lmj/pdb
PDB entry 6lmj coloured by chain, front view.
PDB entry 6lmj coloured by chain, side view.
PDB entry 6lmj coloured by chain, top view.
Source organisms:
Primary publication:
The structural basis of African swine fever virus pA104R binding to DNA and its inhibition by stilbene derivatives.
Liu R, Sun Y, Chai Y, Li S, Li S, Wang L, Su J, Yu S, Yan J, Gao F, Zhang G, Qiu HJ, Gao GF, Qi J, Wang H
Proc Natl Acad Sci U S A 117 11000-11009 (2020)
PMID: 32358196

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-238539 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Viral histone-like protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 110 amino acids
Theoretical weight: 12.46 KDa
Source organism: African swine fever virus
Expression system: Escherichia coli
UniProt:
  • Canonical: P68742 (Residues: 1-104; Coverage: 100%)
Gene names: A104R, BA71V-033
Sequence domains: Bacterial DNA-binding protein
DNA (5'-D(*TP*GP*CP*TP*TP*AP*TP*CP*AP*AP*TP*TP*TP*GP*TP*TP*GP*CP*A)-3') Chains: C, D
Molecule details ›
Chains: C, D
Length: 19 nucleotides
Theoretical weight: 5.8 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U1
Spacegroup: C2
Unit cell:
a: 82.358Å b: 89.095Å c: 53.227Å
α: 90° β: 100.16° γ: 90°
R-values:
R R work R free
0.257 0.256 0.285
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

7 review citations

Current State of Global African Swine Fever Vaccine Development under the Prevalence and Transmission of ASF in China.
Wu et al. (2020)
6 more

2 mentions without citation

The structural basis of African swine fever virus pA104R binding to DNA and its inhibition by stilbene derivatives.
Liu et al. (2020)
1 more