› 6lnw

X-ray diffraction
2.9Å resolution

Crystal structure of accessory secretory protein 1,2 and 3 in Streptococcus pneumoniae

Released:
DOI: 10.2210/pdb6lnw/pdb
PDB entry 6lnw coloured by chain, front view.
PDB entry 6lnw coloured by chain, side view.
PDB entry 6lnw coloured by chain, top view.
Source organism: Streptococcus pneumoniae TIGR4
Primary publication:
Structural and functional insights into the Asp1/2/3 complex mediated secretion of pneumococcal serine-rich repeat protein PsrP.
Guo C, Feng Z, Zuo G, Jiang YL, Zhou CZ, Chen Y, Hou WT
Biochem Biophys Res Commun 524 784-790 (2020)
PMID: 32037091

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-101461 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Accessory secretory protein Asp1 Chain: A
Molecule details ›
Chain: A
Length: 526 amino acids
Theoretical weight: 62.19 KDa
Source organism: Streptococcus pneumoniae TIGR4
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A0H2UR88 (Residues: 1-526; Coverage: 100%)
Gene name: SP_1762
Sequence domains: Accessory Sec system protein Asp1
Accessory secretory protein Asp2 Chain: B
Molecule details ›
Chain: B
Length: 511 amino acids
Theoretical weight: 58.83 KDa
Source organism: Streptococcus pneumoniae TIGR4
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A0H2URA6 (Residues: 1-511; Coverage: 100%)
Gene name: SP_1761
Sequence domains: Accessory Sec system GspB-transporter
Conserved domain protein Chain: C
Molecule details ›
Chain: C
Length: 154 amino acids
Theoretical weight: 18.24 KDa
Source organism: Streptococcus pneumoniae TIGR4
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A0H2URJ0 (Residues: 1-146; Coverage: 100%)
Gene name: SP_1760
Sequence domains: Accessory Sec secretory system ASP3

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P3121
Unit cell:
a: 101.158Å b: 101.158Å c: 196.851Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.199 0.197 0.242
Expression system: Escherichia coli

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