› 6lty

X-ray diffraction
3.28Å resolution

DNA bound antitoxin HigA3

Released:
DOI: 10.2210/pdb6lty/pdb
PDB entry 6lty coloured by chain, front view.
PDB entry 6lty coloured by chain, side view.
PDB entry 6lty coloured by chain, top view.
Source organism: Mycobacterium tuberculosis H37Rv
Primary publication:
Induced DNA bending by unique dimerization of HigA antitoxin.
Park JY, Kim HJ, Pathak C, Yoon HJ, Kim DH, Park SJ, Lee BJ
IUCrJ 7 748-760 (2020)
PMID: 32695421

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-120229 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Putative antitoxin HigA3 Chains: A, B
Molecule details ›
Chains: A, B
Length: 117 amino acids
Theoretical weight: 12.89 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli
UniProt:
  • Canonical: O53333 (Residues: 1-109; Coverage: 100%)
Gene names: P425_03315, RVBD_3183, Rv3183, higA3
Sequence domains: Helix-turn-helix
DNA (5'-D(P*CP*CP*AP*CP*GP*AP*GP*AP*TP*AP*TP*AP*AP*CP*CP*TP*AP*GP*AP*G)-3') Chain: C
Molecule details ›
Chain: C
Length: 20 nucleotides
Theoretical weight: 6.14 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Not provided
DNA (5'-D(P*CP*TP*CP*TP*AP*GP*GP*TP*TP*AP*TP*AP*TP*CP*TP*CP*GP*TP*GP*G)-3') Chain: D
Molecule details ›
Chain: D
Length: 20 nucleotides
Theoretical weight: 6.13 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 5C (4A)
Spacegroup: C2
Unit cell:
a: 74.615Å b: 101.812Å c: 58.027Å
α: 90° β: 90.05° γ: 90°
R-values:
R R work R free
0.28 0.277 0.326
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

3 citation in other articles

The endogenous Coxiella burnetii plasmid encodes a functional toxin-antitoxin system.
Wachter et al. (2022)
2 more

1 mention without citation

Induced DNA bending by unique dimerization of HigA antitoxin.
Park et al. (2020)