› 6lur

X-ray diffraction
2Å resolution

Human PUF60 UHM domain (thioredoxin fusion) in complex with a small molecule binder

Released:
DOI: 10.2210/pdb6lur/pdb
PDB entry 6lur coloured by chain, front view.
PDB entry 6lur coloured by chain, side view.
PDB entry 6lur coloured by chain, top view.
Source organisms:
Primary publication:
Revisiting biomolecular NMR spectroscopy for promoting small-molecule drug discovery.
Hanzawa H, Shimada T, Takahashi M, Takahashi H
J Biomol NMR 74 501-508 (2020)
PMID: 32306215

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141958 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Poly(U)-binding-splicing factor PUF60; Thioredoxin 1 Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 222 amino acids
Theoretical weight: 24.67 KDa
Source organisms: Expression system: Escherichia coli
UniProt:
  • Canonical: P0AA25 (Residues: 1-109; Coverage: 100%)
  • Canonical: Q9UHX1 (Residues: 460-559; Coverage: 18%)
Gene names: FIR, JW5856, PUF60, ROBPI, SIAHBP1, b3781, fipA, trxA, tsnC
Sequence domains: Thioredoxin

Ligands and Environments

1 bound ligand:
Ligand EVU 8 x EVU
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-5A
Spacegroup: P212121
Unit cell:
a: 77.019Å b: 90.59Å c: 288.928Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.203 0.201 0.253
Expression system: Escherichia coli

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Citations

1 review citation

The dynamic nature of the Mre11-Rad50 DNA break repair complex.
Beikzadeh et al. (2021)
2 other articles