› 6lxd

Electron Microscopy
3.9Å resolution

Pri-miRNA bound DROSHA-DGCR8 complex

Released:
DOI: 10.2210/pdb6lxd/pdb
PDB entry 6lxd coloured by chain, front view.
PDB entry 6lxd coloured by chain, side view.
PDB entry 6lxd coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural Basis for pri-miRNA Recognition by Drosha.
Jin W, Wang J, Liu CP, Wang HW, Xu RM
Mol Cell 78 423-433.e5 (2020)
PMID: 32220645
Related structures: EMD-30005

Function and Biology Details

Reaction catalysed: 
Endonucleolytic cleavage to 5'-phosphomonoester.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-235956 (preferred)
Entry contents:
2 distinct polypeptide molecules
1 distinct RNA molecule
Macromolecules (3 distinct):
Ribonuclease 3 Chain: A
Molecule details ›
Chain: A
Length: 990 amino acids
Theoretical weight: 115.39 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q9NRR4 (Residues: 391-1374; Coverage: 72%)
Gene names: DROSHA, RN3, RNASE3L, RNASEN
Sequence domains:
Microprocessor complex subunit DGCR8 Chains: B, C
Molecule details ›
Chains: B, C
Length: 773 amino acids
Theoretical weight: 86.17 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q8WYQ5 (Residues: 1-773; Coverage: 100%)
Gene names: C22orf12, DGCR8, DGCRK6, LP4941
Sequence domains: Double-stranded RNA binding motif
RNA (102-mer) Chain: D
Molecule details ›
Chain: D
Length: 102 nucleotides
Theoretical weight: 32.76 KDa
Sequence domains: mir-15 microRNA precursor family

Ligands and Environments

1 bound ligand:
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.9Å
Relevant EMDB volumes: EMD-30005
Expression system: Spodoptera frugiperda

Quick links

Citations

15 review citations

Reexamining assumptions about miRNA-guided gene silencing.
Kilikevicius et al. (2022)
14 more

4 mentions without citation

Secondary structure RNA elements control the cleavage activity of DICER.
Nguyen et al. (2022)
3 more